Unlike the existing LC-inlet solution via chemical oxidation, the iso CHROM LC works with high-temperature combustion. It quantitatively converts organic carbon to CO2 and organic nitrogen to N2. Thus, for the first time, it is possible to alternatively perform stable nitrogen isotope analysis.
In addition, the easy-to-use iso CHROM LC shows outstanding precision, accuracy, and linearity without significant peak broadening that can diminish the chromatographic performance. In continuous operation, virtually all peaks in a chromatogram can be analyzed for the stable isotope ratios of carbon or nitrogen.
The iso CHROM LC combines the proven Elementar high-temperature combustion technology with straight-forward combustion gas purification. This results in unrivaled low peak broadening that brings the chromatographic resolution of the HPLC system virtually unchanged to the mass spectrometer. This makes the iso CHROM LC the first choice of any complex separation tasks in LC-IRMS.
No isotope fractionation
Thanks to the fast and complete conversion process of the high-temperature combustion, no isotope fractionation is observed. Period. This results in precise, high-quality data that can be trusted to represent the actual isotope ratios of a wide variety of samples.
Stable nitrogen isotope analysis
For the first time, it is possible to analyze stable nitrogen isotopes with LC-IRMS. In combination with stable carbon isotope analysis, this opens an entire new world of possibilities in studying non-volatile or thermolabile organic compounds that contain nitrogen. No derivatization prior to analysis is required.
The iso CHROM LC can be connected to any HPLC system to receive a sample liquid stream for conversion to CO2 and N2. Alternatively, direct injection of samples can be carried out as well to perform both bulk isotopic analysis and compound-specific analysis using the same system.